Human Aortic Endothelial Cells

Description

Human Aortic Endothelial Cells (HAoEC) are primary cells isolated from the human ascending (thoracic) and descending (abdominal) aorta. Following primary culture, these cells are cryopreserved. The cells exhibit characteristic cobblestone appearance with prominent nuclei. During proliferation, the cells become small and evenly sized, and demonstrate high mitotic index, while maintaining purity (α-SMA-negative). Functionally, these cells regulate vascular tone through the secretion of nitric oxide (NO) and prostacyclin, maintain the integrity of the blood-tissue barrier, and mediate immune responses by expressing ICAM-1/VCAM-1, and participate in angiogenesis in aortic endothelium. They are critically implicated in atherosclerosis, aortic aneurysm, hypertension, and diabetic vasculopathy. Cryopreserved at early passage, these cells retain native functionality but should not undergo repeated freeze-thaw cycles to maintain optimal performance. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.