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Human Umbilical Vein Endothelial Cells

Product Name

Human Umbilical Vein Endothelial Cells

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Product Code

HUVEC

Cat.No

ABC-TC3848

Species

Human

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Human Umbilical Vein Endothelial Cells (HUVECs) are cells from the vein of umbilical cord. They have cobblestone morphology with large dark nuclei when observed under light microscope. HUVECs are the most commonly used source of endothelial cells due to their easy accessibility and affordability in price.

How to obtain human umbilical vein endothelial cells

HUVEC cells can be obtained from discorded umbilical cord after childbirth by enzymatic digestion of the lumen. They express many important endothelial signal molecules, for example von Willebrand factor (vWF), which is associated with vascular homeostasis regulation, blood platelets mediation and blood vessel wall tone.

Why choose HUVECs from AcceGen

The isolation of human umbilical vein endothelial cells in AcceGen is from umbilical vein vascular wall by collagenase digestion and culture. Each vial contains 5*10^5 cells with high quality. Cells are cryopreserved at the end of primary culture in cell culture media kits with 10% DMSO and delivered frozen.  HUVECs are guaranteed a further expansion of the population by 15 times when cultured with AcceGen recommended culture medium and culture protocols.

 

HUVECs are tested for specific antibodies against vWF/Factor VIII, CD31 and uptake of Dil-Ac-LDL by immunofluorescent approach. All cells lots are detected negative for the presence of HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

 

AcceGen recommends handling HUVECs with appropriate biosafety procedures due to its human origin. If not using HUVECs immediately, transfer cells to liquid nitrogen (-180) until ready for use.

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Human umbilical vein endothelial cells culture can be used as a laboratory model for studying vasculature and angiogenesis. They are also good cell source to build scaffolds and facilitate vascularisation in tissue engineering. HUVECs can be used to characterize endothelial cell stress response towards physiological and pathophysiological stimuli and build 3D model system to study cell-cell interaction, cell migration, tumour angiogenesis as well as many other researches.

Infected Primary HUVECs and Immortalized HUVECs are also available in AcceGen.

Key Features

-Backed by AcceGen advanced technology

-Cryopreserved for highest viability and plating efficiency

-Quality-tested for accurate results

Product Type

Vascular Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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