Home > Products > Tumor Cell Lines > Human Tumor Cell Lines >
MKN-1 | ||||
---|---|---|---|---|
Product Name | MKN-1 | |||
Price | Get Quote | |||
Product Code | MKN-1; MKN 1 | |||
Cat.No | ABC-TC0685 | Species | Human | |
Size/Quantity | 1 vial | Biosafety Level | 1 | |
Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
Description |
Why choose MKN-1 from AcceGen? Accegen offers every customer MKN-1 with the best quality and viability. The production of MKN-1 is conducted using standardized culture system and procedures. Before delivery, MKN-1 passes rigorous tests, including the parameters assessing cell morphology and viability, and negative performance for mycoplasma, bacteria, yeast, and fungi. | |||
Source Organ | Stomach | |||
Recommended Medium And Supplement | RPMI 1640 + 10% FBS | |||
Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
Application | FOR RESEARCH USE ONLY Due to the rarity of human gastric adenosquamous carcinoma, limited clinical options are available to these patients. The utilization of MKN-1 in the scientific reports might unravel the cell proliferative properties of gastric carcinomas. MKN-1 uncovered that E-cadherin can be silenced by promoter CpG hypermethylation. MKN-1 can also used to discover inhibitory effects on tumor invasion. For example, eupatilin and exogenous RUNX3 has the potential to inhibit gastric cancer cell proliferation in MKN-1. | |||
Growth Conditions | 37 ℃, 5% CO2 | |||
Questionsthree | How do I assess the health and viability of MKN-1 cells? | |||
Product Type | Human Gastric Cancer Cell Lines | |||
Product Image |
Frequently Asked Questions
How do I maintain the morphology of MKN-1 cells?
Morphology: MKN-1 cells are epithelial in nature and should appear as adherent, polygonal cells. They should form a monolayer without significant clumping or detachment.
Monitoring: Regularly observe cells under a microscope to ensure they retain their characteristic morphology. Abnormalities in shape or detachment may indicate issues with culture conditions.
How do I manage cell doubling time?
Expected Doubling Time: MKN-1 cells typically have a doubling time of 24-36 hours under optimal conditions.
Troubleshooting Slow Growth
Nutrient Depletion: Ensure medium is changed every 2-3 days.
Cell Density: Avoid over-confluency. Split cells when they reach 70-80% confluence.
Contamination: Regularly check for microbial contamination.
Medium Quality: Use high-quality FBS and RPMI 1640 medium. Ensure they are not expired or contaminated.
Incubator Conditions: Confirm stable temperature and CO₂ levels in the incubator.How do I assess the health and viability of MKN-1 cells?
Viability Assays: Use trypan blue exclusion or MTT assay to determine cell viability.
Morphological Assessment: Regularly observe under a microscope for normal morphology.
Growth Curves: Plot cell growth over time to ensure consistent doubling time and healthy proliferation rates.
- ONLINE INQUIRY
- PRODUCT REVIEWS
Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.