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MKN-45

Product Name

MKN-45

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Product Code

MKN-45; MKN 45

Cat.No

ABC-TC0687

Species

Human

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

MKN-45 is a human gastric cancer cell line derived from poorly differentiated gastric adenocarcinoma. MKN-45 cells have been used extensively in gastric cancer development study and therapeutic agent assessment. MKN-45 gastric cancer cells express wild-type p53 and are characterized by homozygous deletion of p16CDKN2/MTS1/INK4A and p15MTS2, as well as amplification of c-met oncogene and promoter mutation of E-cadherin. MKN-45 cells exhibit both normal and metaplastic gastric mucosa phenotype with preserved differentiation capacity.

Why choose MKN-45 from AcceGen?

MKN-45 is derived from poorly differentiated gastric adenocarcinoma of a 62-year-old woman. Cells are cryopreserved to ensure the best viability. Each vial contains more than 1×10^6 cells in 1ml volume. MKN-45 cells are characterized by spindle-shaped or oval-shaped morphology and present a hypertriploid karyotype with 8% polyploidy. Subculture cells at a split ratio of 1:3 to 1:5 every 5-10 days using trypsin/EDTA.

Source Organ

Liver

Quality Control

All cell lots test negative for HIV-1, HBV, HCV, mycoplasma, yeast, fungi, and other pathogens.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Bezdan, D., Grigorev, K., Meydan, C., Vatter, F.A.P., Cioffi, M., Rao, V., MacKay, M., Nakahira, K., Burnham, P., Afshinnekoo, E. and Westover, C., 2020. Cell-free DNA (cfDNA) and exosome profiling from a year-long human spaceflight reveals circulating biomarkers. IScience, p.101844.

Application

For research use only

MKN-45 cells provide a useful model for studying the biology and characteristic of gastric cancer. They can also be used to screen for potential drugs or underlying mechanism of gastric cancer and the immunological pathway associated with it.

Key Features

– Backed by AcceGen advanced technology

– Cryopreserved for highest viability and plating efficiency

– Quality-guaranteed for accurate results

– Timely technical support for best experimental outcomes

Growth Conditions

37 ℃, 5% CO2

QuestionsthreeHow do I passage MKN-45 cells?
Product Type

Human Gastric Cancer Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial

Frequently Asked Questions

  • What are MKN-45 cells?

    MKN-45 cells are a human gastric adenocarcinoma cell line commonly used in gastric cancer research, drug screening, and anti-cancer therapy studies.

  • How do I start culturing MKN-45 cells?

    Thawing cells: Quickly thaw frozen cells in a 37°C water bath for no more than 2 minutes. Gradually transfer the cells into pre-warmed RPMI 1640 complete medium with 10% fetal bovine serum (FBS).

    Seeding cells: Transfer the cell suspension to a culture flask and incubate at 37°C in a 5% CO₂ incubator.

  • How do I passage MKN-45 cells?

    Collecting cells:
    Suspension cells: MKN-45 cells are partially adherent and partially in suspension. Gently shake the flask to evenly distribute the suspension cells.
    Adherent cells: Use trypsin to detach adherent cells. First, remove the old medium and add a small amount of trypsin solution to cover the cells, then incubate at 37°C for 1-2 minutes.

     

    Stopping trypsinization: Add fresh pre-warmed complete medium to neutralize the trypsin, and gently pipette up and down to detach the cells completely.

     

    Centrifugation: Transfer the cell suspension to a centrifuge tube and centrifuge at 300xg for 5 minutes.

     

    Resuspending cells: Carefully remove the supernatant and resuspend the cell pellet in pre-warmed complete medium.

     

    Seeding new flasks: Dilute the cell suspension to the desired density and seed into new culture flasks.

  • How do I perform morphological observation of cells?

    Regularly observe cell morphology under a microscope, noting any changes in shape, confluency, and the presence of abnormal cells or contaminants.

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