The inner layer of stomach is the gastric mucosa covered by mucous, which is secreted by a layer of simple columnar epithelial cells lining the lumen of stomach. The gastric epithelium invaginate to form gastric pits that connect to various glands that synthesis and secrete stomach juice.
Types of epithelial cells in stomach Stomach epithelial cells consists of mucous cells, stomach epithelium chief cells, parietal cells and gastrin cells (G cells) that extends from stomach surface into gastric pits and glands. Injury, inflammation, or bacterial infection, especially Helicobacter pylori infection, on stomach can stimulate stomach epithelial cells proliferation and renewal.
Why choose Human Gastric Epithelial cells from AcceGen? AcceGen Human gastric epithelial cells are isolated from normal human stomach tissue under authorization. Shortly after isolation, cells are cryopreserved at passage 3 in AcceGen cell culture media to ensure best quality. Each vial contains >5×10^5 cells in 1 ml volume. Our gastric epithelial cells culture can further expand for 2-4 passages at a split ration of 1:2 or 1:3 following AcceGen recommended protocol and culture conditions. Cells can form coherent monolayer and develop tight junction in AcceGen cell culture media with expression of epithelial marker cytokeratin and junction-associated marker E-cadherin or ZO-1.
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
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For research use only AcceGen human gastric epithelial cells provide an excellent model for study gastrointestinal epithelial function and pathophysiology in vitro. The gastric epithelial cells can be used to study pathogenesis of H. pylori, screen effective antiulcer drugs, measure permeability and study gastric related digestive functions.
-Backed by AcceGen advanced technology -Cryopreserved for highest viability and plating efficiency -Quality-tested for accurate results
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