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C57BL/6 Mouse Retinal Microvascular Endothelial Cells

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Product Name

C57BL/6 Mouse Retinal Microvascular Endothelial Cells

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Cat.No

ABC-TC3241

Species

C57BL/6 Mouse

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

C57BL/6 Mouse Retinal Microvascular Endothelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Retinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen.
Mouse Retinal Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Disease

Normal

Source Organ

Retina

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Endothelial

Growth Mode

Adherent

Product Type

Mouse Primary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial

Frequently Asked Questions

  • What is C57BL/6 Mouse Retinal Microvascular Endothelial Cells?

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells are isolated from retinal microvasculature, characterized by barrier function and angiogenesis, and specifically express VE-cadherin and CD31.

  • Application of C57BL/6 Mouse Retinal Microvascular Endothelial Cells

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells are used in research to study retinal vascular biology, blood-retinal barrier function, and angiogenesis. They are vital for understanding diabetic retinopathy, age-related macular degeneration, and other retinal diseases. These cells enable the evaluation of new therapeutic agents and the exploration of molecular pathways involved in retinal vascular health. Their application in developing treatments for retinal disorders holds significant promise for advancing ocular disease management and therapy.

  • What are the cell culture conditions for C57BL/6 Mouse Retinal Microvascular Endothelial Cells?

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen.

  • What happens to C57BL/6 Mouse Retinal Microvascular Endothelial Cells during in vitro culture?

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells initially adhere to the culture surface as single cells or small clusters, exhibiting a spindle-shaped morphology. As they proliferate, they form a monolayer with a cobblestone appearance, resembling typical endothelial cell morphology. Over time, endothelial cells may display elongated or polygonal shapes depending on their confluence and passage number. Additionally, they may develop tube-like structures when cultured in appropriate angiogenic conditions, mimicking their in vivo behavior.

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