Human Aortic Smooth Muscle Cells-Diabetic Type I

Description

Human Aortic Smooth Muscle Cells-Diabetic Type I are primary cells isolated from the descending aorta of clinically confirmed Type 1 diabetic donors. Cryopreserved at early passage, these cells exhibit characteristic spindle-shaped morphology with hill-and-valley growth patterns. These cells serve as a classic in vitro model for investigating smooth muscle cell response to hyperglycemia and diabetic vascular aging in smooth muscle cells, and have been identified as critical contributors to diabetic macroangiopathy, including accelerated atherosclerosis, aortic aneurysm vulnerability, and microvascular complications like nephropathy due to AGE-mediated medial degeneration and ROS overproduction. This population expresses disease-relevant surface markers CD44 and RAGE. They also exhibit upregulated MMP-9 and LOX-1 expression alongside suppressed contractile markers, while maintaining diploid karyotype stability. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.