Human Astrocytes-Cerebella

Description

Human Astrocytes-Cerebella (HA-c) are primary cells isolated from human cerebellum. Following primary culture, these cells are cryopreserved. These specialized glial cells demonstrate characteristic astrocytic morphology and function, playing critical roles in cerebellar homeostasis through neuronal support, synaptic regulation, immune response of cerebellar astrocytes, and injury response. Astrocytes are also implicated in various pathological processes. Impairment of normal astrocyte functions during stroke and other insults has been shown to critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is influenced by astrocyte surface molecule expression and trophic factor release. Strong expression of glial fibrillary acidic protein (GFAP) is confirmed, validating their astrocyte identity. For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.