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Transfected Stable Cell Lines

Human BRAF (L597Q) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human BRAF (L597Q) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0278C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human BRAF (L597Q) BAF3 Cell Line is a genetically engineered model derived from murine pro-B Ba/F3 parental cells based on customers’ requirement. BRAF (L597Q) BAF3 overexpression cell line is generated by stable integration of exogenous human BRAF containing the L597Q mutation using lentiviral transduction. Overexpression clone is validated at gene level by qRT-PCR.
Target
BRAF encodes a serine/threonine-protein kinase that activates MEK and ERK in the MAPK signaling pathway. The L597Q mutation is a kinase-activating variant found in cancers such as melanoma and colorectal cancer. It contributes to aberrant cell growth and transformation. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

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Application

  • For research use only

  • The BRAF (L597Q) BAF3 Cell Line is a robust platform for dissecting mutation-specific BRAF signaling, drug response profiling, and evaluation of RAF and MEK inhibitors in cancers with non-V600 BRAF alterations.

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