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Primary Cells

Human Intestinal Epithelial Cells

  • BSL

    1

  • 620
cryopreserved InEpC are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib).
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Home | Primary Cells | Human Primary Cells | Digestive System Primary Cells | Human Intestinal Epithelial Cells
Product Code

InEpC
Species

Human
Cat.No

ABC-TC3879
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Epithelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Intestine
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Intestinal Cells
Key Features

-Backed by AcceGen advanced technology
-Ship cryopreserved cells in dry ice
-Quality-tested for accurate results

Description

Human Intestinal Epithelial Cells
Human Intestinal Epithelial Cells are derived from human intestinal tissue. These cells exhibit a polygonal morphology in vitro and form a polarized monolayer.Beyond their fundamental roles in nutrient absorption and mucus secretion, IECs produce an array of bioactive molecules including antimicrobial peptides (defensins, cathelicidins), immunoregulatory cytokines (IL-10, TGF-β), and enteroendocrine hormones (serotonin, GLP-1) that collectively maintain intestinal homeostasis. These cells form tight junctions and adherens junctions to create a selective barrier, while also sampling luminal contents through specialized microfold (M) cells.They express key epithelial junctional proteins such as ZO-1, claudins, and occludin, essential for barrier integrity. Their dysfunction is implicated in various gastrointestinal pathologies including inflammatory bowel disease (through barrier disruption and aberrant immune activation), colorectal cancer (via Wnt/β-catenin signaling dysregulation), and celiac disease (where gliadin triggers inappropriate immune responses)
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Citation

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Application

  • Human Intestinal Epithelial Cells can be used to establish an available epithelial cell culture system to investigate the exact pathogenesis of gastrointestinal diseases. They can also be used to build infection models that help better understand the host-parasite interactions, extracellular matrix–cell interactions, microbiota interfaces, and population screens.

Frequently Asked Questions

  • What are Human Intestinal Epithelial Cells?

    Human Intestinal Epithelial Cells (IECs) are the cells that line the surface of the intestines. They form a barrier between the interior of the intestine and the rest of the body, playing a crucial role in nutrient absorption, immune function, and maintaining the integrity of the intestinal lining.

  • Where are Human Intestinal Epithelial Cells isolated from?

    Human intestinal epithelial cells are isolated from the entire intestine, including the small intestine and the large intestine

  • What functions do Human Intestinal Epithelial Cells perform in the gut?

    Human Intestinal Epithelial Cells are responsible for several key functions, including absorption of nutrients and water, secretion of digestive enzymes and mucus, maintenance of the intestinal barrier, and participation in immune responses by interacting with gut-associated lymphoid tissues.

  • How many times can Human Intestinal Epithelial Cells be passaged?

    Human Intestinal Epithelial Cells can typically be passaged 3-5 times. However, with each passage, the characteristics of the epithelial cells gradually change. Therefore, it is generally recommended to use these cells within three passages.

  • What are Human Intestinal Epithelial Cells used for in research?

    Human Intestinal Epithelial Cells are used in research to study intestinal physiology, pathophysiology, and diseases such as inflammatory bowel disease (IBD), colorectal cancer, and infections. They are also used in drug absorption and toxicity studies, as well as in the development of new therapeutic strategies.

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