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Primary Cells

Human Orbital Fibroblasts

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Home | Primary Cells | Human Primary Cells | Nervous System Primary Cells | Human Orbital Fibroblasts
Species

Human
Cat.No

ABC-TC077G
Quality Control

All cells test negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Fibroblast
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Orbital Tissue
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Orbital Cells
Passage Number

1

Description

Human orbital fibroblasts are derived from human orbital connective tissue. Following the primary culture, these cells are cryopreserved. Orbital fibroblasts contribute to the structural maintenance of orbital tissue. Studies have shown that these cells are implicated in Graves’ ophthalmopathy (GO). In vitro, human orbital fibroblasts exhibit distinct responses to stimulation, including binding to endothelin and producing high levels of IL-6 upon treatment with IL-1β. These cells should be handled carefully to avoid repeated freezing and thawing during culture.

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Citation

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Application

  • Human orbital fibroblasts are a vital model for investigating diseases related to the connective tissue of the orbit, such as Graves’ ophthalmopathy (GO), also known as thyroid-associated ophthalmopathy (TAO). These fibroblasts enable researchers to study the significant inflammation characteristic of these conditions, where tissues become infiltrated by activated T lymphocytes and mast cells, leading to the excessive accumulation of hyaluronan. Using human orbital fibroblasts allows the exploration of communication pathways between fibroblasts and immune cells, providing insights into the mechanisms driving inflammation and disease progression, which could inform potential therapeutic approaches.

Frequently Asked Questions

  • What are human orbital fibroblasts, and what function do they serve?

    Human orbital fibroblasts are specialized cells found in the connective tissue of the orbit. They help maintain the structural integrity of the orbit, produce extracellular matrix components, and contribute to tissue repair and remodeling. They are also involved in various physiological and pathological processes, including inflammation and fibrosis.

  • How are human orbital fibroblasts typically obtained and cultured for research purposes?

    Human orbital fibroblasts are usually obtained from biopsy samples of orbital tissue. For culturing, these cells are grown in specialized media that support their growth and maintain their functionality. The media often contain nutrients, growth factors, and supplements to mimic the natural environment of orbital fibroblasts.

  • What are the main applications of human orbital fibroblasts in scientific research?

    Human orbital fibroblasts are used to study conditions like thyroid eye disease (Graves’ orbitopathy), orbital inflammation, and fibrosis. They are important for developing and testing new treatments for these conditions and for understanding the mechanisms of tissue remodeling and scarring in the orbital region.

  • What are the key characteristics when culturing human orbital fibroblasts?

    Key characteristics include their spindle-shaped morphology, proliferation rate, and expression of specific markers like vimentin and fibronectin. They should show healthy growth patterns, proper cell-to-cell interactions, and responsiveness to changes in media composition or the introduction of pharmacological agents.

  • What are the main challenges in culturing and experimenting with human orbital fibroblasts?

    Challenges include maintaining their viability and functionality in vitro, replicating the complex natural environment, and preventing contamination. Variability between primary fibroblast cultures from different donors can affect experimental consistency. Another challenge is the potential for fibroblasts to become myofibroblasts under certain conditions, which can change their behavior and affect experimental results.

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