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Primary Cells

Human Osteoblasts, Femoral

  • For research use only

Cat No.

ABC-TC3740
Product Type

Osteoblasts
Cell Type

Osteoblast
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Bone
Disease

Normal
Storage

Liquid Nitrogen

Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells.

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  • Human Osteoblasts, Femoral
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Description

Human Osteoblasts, Femoral (HO f) are primary bone-forming cells isolated from adult human femoral cortical tissue. These cells exhibit the classic cuboidal morphology and actively deposit extracellular matrix components, including type I collagen (COL1A1/COL1A2) and non-collagenous proteins essential for mineralization. HO f cells are cryopreserved at an early passage. They express typical osteoblastic markers, including tissue-nonspecific alkaline phosphatase (ALPL), osteocalcin (BGLAP), and bone sialoprotein (IBSP). In early culture, HO f show high ALP activity and form mineralized osteoid in vitro, reflecting physiological bone matrix formation. As non-immortalized primary cells, they have finite proliferative capacity and are not suitable for long-term passaging. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.
Product Code

Human Femoral Osteoblasts, Femur Derived Osteoblasts Human, Femoral Bone Osteoblasts, hOBs Femur, Primary Femur Osteoblasts
Species

Human
Cat.No

ABC-TC3740
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

Osteoblast
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Bone
Disease

Normal
Storage

Liquid Nitrogen
Product Type

Osteoblasts
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • Human femoral osteoblasts are ideal for studying osteogenesis, bone biomaterial interactions, and endocrine modulation of bone remodeling. They are commonly applied in assays measuring ALP activity, osteocalcin secretion, collagen synthesis, and mineral deposition over time, providing critical data for both mechanistic studies and preclinical drug development. These cells are valuable for evaluating the efficacy of osteoporosis therapeutics, investigating the impact of hormones and cytokines on bone formation, and testing orthopedic implant biocompatibility. Their human origin and retention of native osteoblastic functions make them superior to immortalized lines for translational studies in bone biology.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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