Human Periodontal Ligament Fibroblasts

Description

Human Periodontal Ligament Fibroblasts (PLFs) are derived from human periodontal ligament tissue. They are the predominant cell type in periodontal ligament and play an important role in the development, function, and regeneration of periodontal connective tissues. PLFs are considered multipotent cells and involved in the synthesis and phagocytosis of collagen and matrix components, secretion of cytokines, as well as osteoclastogenesis. While morphologically resembling gingival fibroblasts, PLFs stand apart in their unique biological responses, matrix protein expression, and elevated alkaline phosphatase activity — a signature of their bone remodeling prowess. PLFs express typical mesenchymal markers such as vimentin, STRO-1, and alkaline phosphatase (ALP).Their unique ability to differentiate into osteoblast-like cells under certain conditions highlights their critical role in maintaining periodontal homeostasis and supporting bone remodeling.

Application

• Human Periodontal Ligament Fibroblasts can be used to explore the pathogenesis of periodontitis, drug screening and tissue engineering research. For example, by simulating an inflammatory environment, the effects of cytokines (such as IL-6, TNF-α) on periodontal tissue destruction can be analyzed, or the effects of new biomaterials (such as hydrogels, scaffolds) on cell adhesion and proliferation can be evaluated