Human Peripheral Blood Mononuclear Cells, Fresh

Description

Human Peripheral Blood Mononuclear Cells (PBMCs), Fresh are mononuclear cell populations isolated from healthy human peripheral blood, which are enriched from whole blood by density gradient centrifugation (e.g., Ficoll-Paque). These freshly isolated primary cells are typically not cultured or passaged. PBMCs mainly include lymphocytes (T cells, B cells, NK cells) and monocytes. PBMCs are the core executors of innate and adaptive immune responses: T cells and B cells mediate specific immunity, NK cells exert killing functions, and monocytes can differentiate into macrophages or dendritic cells, participating in antigen presentation and inflammation regulation. These cells express common immune markers such as CD3 (T cells), CD19 (B cells), CD56 (NK cells), and CD14 (monocytes). Their abnormal activation or dysfunction is associated with a variety of diseases, such as T cell overactivation leading to graft-versus-host disease (GVHD), while monocyte infiltration promotes atherosclerotic plaque formation. As freshly isolated mononuclear cells from human peripheral blood, these peripheral mononuclear cells represent a key subset of peripheral blood cells for ex vivo immune monitoring and functional assays.