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Human Peripheral Blood Mononuclear Cells, Fresh

Product Name

Human Peripheral Blood Mononuclear Cells, Fresh

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Cat.No

ABC-TC3727

Species

Human

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.
Samples from each donor are tested via PCR to confirm non-reactivity.

Disease

Normal

Source Organ

Peripheral Blood

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Citation Guide

When you publish your research, please cite our product as "AcceGen Biotech Cat.# XXX-0000". In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only.
Peripheral blood mononuclear cells (PBMCs) are widely used in experimental studies. They are isolated from peripheral blood and are often employed to assess immune responses. PBMCs have wide application in many hot topics, for example, multi-omics analysis of PBMCs based on CITE-seq data, in vitro differentiation of PBMCs into cardiomyocytes or vascular-associated cells. Our products are ideal for establishing an in vitro PBMC differentiation system that allows you to combine molecular and cytological approaches to study the differentiation mechanisms of PBMCs.

Growth Conditions

37 ℃, 5% CO2

Cell Type

Mononuclear Cell

Growth Mode

Adherent

Product Type

Normal Human Peripheral Blood Mononuclear Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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