Human urothelial cells are primary epithelial cells isolated from normal bladder tissue of human donors. These cells exhibit epithelial-like morphology with adhere to culture surfaces, forming a stratified barrier that lines the urinary tract. Typically cryopreserved after initial culture, they retain limited proliferative capacity. Functionally, urothelial cells maintain urinary barrier integrity through uroplakin-rich apical membranes enriched with uroplakins and the formation of tight junctions. Key differentiation markers include cytokeratin 20 (CK20), expressed in mature umbrella cells and uroplakins (UPK1/2) . These cells are intrinsically linked to urothelial carcinoma pathogenesis of urothelial carcinoma and chronic inflammatory condistion such as interstitial cystitis. 
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