IL10 Reporter HEK-293 Cell Line

Description

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine with important immunoregulatory functions. It inhibits the expression of inflammatory cytokines like TNF-α, IL-6, and IL-1 by activating macrophages. In its functional form, IL-10 exists as a homodimer.IL-10 signaling is transmitted through a receptor complex composed of two IL-10 receptor 1 and two IL-10 receptor 2 proteins. Therefore, the functional receptor consists of four IL-10 receptor molecules.Upon binding IL-10R1 (IL-10Rα), the IL-10R1 subunit interacts with IL-10R2 (IL-10Rβ). The receptor complex phosphorylates the cytoplasmic tails of IL-10 R1 and IL-10 R2 via JAK1 and Tyk2, ultimately leading to phosphorylation of STAT dimers. The phosphorylated STAT dimers then translocate to the nucleus.The IL10 Reporter HEK-293 Cell Line is a luciferase reporter cell line constructed based on the JAK-STAT signaling pathway. When IL-10 binds to IL-10R1 and subsequently to IL-10R2, the receptor complex is phosphorylated by JAK1 and Tyk2. This results in phosphorylation and translocation of STAT dimers, leading to the expression of luciferase. Luciferase readings represent the activation effect of the signaling pathway, making it useful for in vitro evaluation of IL-10 related drugs.