Immortalized Human Endometrial Epithelial Cells is an immortalized epithelial cell line derived from the endometrial tissue of a healthy adult female donor, immortalized with SV40 large T antigen. These cells display typical epithelial characteristics with a polygonal shape and grow adherently in standard culture conditions (37°C, 5% CO₂) in epithelial cell culture medium supplemented with 10% FBS. The cells express high levels of epithelial markers such as EpCam, Keratin 17, and Keratin 19, and stem cell-associated proteins like OCT3/4 and SSEA-1, but do not express CD13. The expression levels of SOX2, P63, c-Myc, and CD117 decline with passaging. While karyotype and mutation profiling have not been reported, the cells are non-tumorigenic and non-metastatic.
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