Immortalized Human Intestine Fibroblasts

Description

Immortalized Human Intestine Fibroblasts are derived from normal human intestinal tissue and modified—typically via hTERT or SV40 large T antigen—to overcome replicative senescence while preserving physiological functions. These spindle-shaped, adherent cells contribute to epithelial support, extracellular matrix remodeling, and mucosal immune regulation. They consistently express characteristic fibroblast markers including vimentin, collagen type I, and fibronectin, while also demonstrating functional responsiveness through secretion of cytokines like IL-6 and TGF-β, particularly under inflammatory stimulation. By facilitating epithelial-mesenchymal crosstalk, these human fibroblast cell lines serve as a robust model for investigating intestinal fibrosis, inflammatory bowel diseases (IBD), and tumor–stroma interactions in colorectal cancer. Their stable phenotype and extended lifespan make them well-suited for long-term co-culture, mechanistic studies, and drug screening applications.