For research use only
| Cat No. | ABC-TC232S |
| Product Type | Rat Pancreas Cancer Cell Lines |
| Cell Type | β Cell |
| Species | Rattus Norvegicus |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Pancreas |
| Product Code | INS1 |
INS-1 rat insulinoma cells express insulin, respond to glucose, and model pancreatic β-cell function and diabetes using mature and bi-hormonal subtypes.
INS-1 is a radiation‑induced rat insulinoma cell line derived from a 2-year-old male rat (Rattus norvegicus) following X‑ray exposure. INS-1 exhibits adherent, beta‑cell‑like morphology, adherent culture properties and robust glucose‑stimulated insulin secretion, characteristic of pancreatic β-cells. INS‑1 cells maintain bi‑hormonal expression, co‑expressing insulin and low levels of glucagon, reflecting an immature endocrine phenotype. Gene expression profiling reveals reduced Nkx6. 1 and absence of mature α‑cell markers, but active expression of prohormone convertases PC1/3 and PC2. Its well‑defined physiology makes it a suitable cell line to investigate pancreatic islet beta-cell function and diabetes.
| Product Code | INS1 |
| Species | Rattus Norvegicus |
| Cat.No | ABC-TC232S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | β Cell |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Pancreas |
| Biosafety Level | 2 |
| Storage | Liquid Nitrogen |
| Product Type | Rat Pancreas Cancer Cell Lines |
The INS-1 cell line, renowned for its stability and preserved differentiation, serves as an ideal model to investigate diverse facets of pancreatic β‑cell biology, including insulin secretion, glucose metabolism, and dysfunction modeling in diabetes research. It is extensively employed in screening and evaluation of anti‑diabetic compounds and in investigating pathways regulating insulin gene expression and secretion. Moreover, INS-1 cells offer valuable insights into the expression and regulation of proglucagon-derived peptides within primary islets, unraveling their responses to a range of metabolic stimuli.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
The percentage of HMB-45 expression in INS-1 Cell Line was detected by flow cytometry to be approximately 92.8%.
The INS-1 cell line is an immortalized cells induced by radiation, isolated from pancreas of an x-ray-created transplantable insulinoma rats.
The culture medium recommended for INS- 1 cells is composed of 1640 medium supplemented with 2.0 g/L Glucose, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium pyruvate, 1.5 g/L NaHCO3 to support optimal cell growth and maintenance, add 10% FBS.
The sodium pyruvate, HEPES, and mercaptoethanol should be added at the specified concentration for cell growth.
The INS-1 cell line show weak attachment at the beginning of the recovery, usually take 24-48 hours without changing medium. In the first few days, observing floating cells is normal. This cell also grow slowly, may form attached clumps. Please be patient and take care. Normally 3 days after thawing, the cells can be trypsinized and plated on 2 flasks to expand.
