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| Product Code | INS1 |
| Species | Rattus Norvegicus |
| Cat.No | ABC-TC232S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | β Cell |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Pancreas |
| Storage | Liquid Nitrogen |
| Product Type | Rat Pancreas Cancer Cell Lines |
INS-1 is a rat insulinoma cell line that was originally isolated from pancreas tissue of the x-ray-induced insulinoma in a 2-year-old male rat. INS-1 exhibits the beta-cell-like morphology and adherent culture properties. These cells express a high level of insulin and are sensitive to glucose fluctuations. The INS-1 cells are heterogeneous, consisting of both mature insulin-positive cells and immature bi-hormonal cells that co-express insulin and glucagon. Therefore, INS-1 is a suitable cell line to investigate pancreatic islet beta-cell function and diabetes.
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The INS-1 cell line, renowned for its stability and preserved differentiation, serves as an ideal model to investigate diverse facets of beta-cell activity. It finds extensive utility in deciphering insulin secretion mechanisms, particularly excelling as a tool to explore glucose-triggered insulin release. Moreover, INS-1 cells offer valuable insights into the expression and regulation of proglucagon-derived peptides within primary islets, unraveling their responses to a range of metabolic stimuli.
The INS-1 cell line is an immortalized cells induced by radiation, isolated from pancreas of an x-ray-created transplantable insulinoma rats.
The culture medium recommended for INS- 1 cells is composed of 1640 medium supplemented with 2.0 g/L Glucose, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium pyruvate, 1.5 g/L NaHCO3 to support optimal cell growth and maintenance, add 10% FBS.
The sodium pyruvate, HEPES, and mercaptoethanol should be added at the specified concentration for cell growth.
The INS-1 cell line show weak attachment at the beginning of the recovery, usually take 24-48 hours without changing medium. In the first few days, observing floating cells is normal. This cell also grow slowly, may form attached clumps. Please be patient and take care. Normally 3 days after thawing, the cells can be trypsinized and plated on 2 flasks to expand.
