IRF-Lucia Reporter Raw264.7 Cell Line

Description

IRF-Lucia Reporter Raw264.7 Cell Line is a genetically engineered macrophage model derived from murine Raw264.7 cells, designed for sensitive and quantitative monitoring of IRF (Interferon Regulatory Factor) signaling pathway activation. This stable cell line features an integrated IRF-responsive Lucia luciferase reporter system that enables high-sensitivity detection of IRF-mediated transcriptional activity through bioluminescent readouts. The cell line maintains its monocyte/macrophage origin with characteristic adherent morphology while preserving key macrophage functions including cytokine production and phagocytic activity. The Lucia luciferase reporter system offers superior stability and sensitivity compared to conventional luciferase, making this cell line ideal for studying innate immune responses, interferon signaling, and screening immunomodulatory compounds. Key macrophage markers including CD14 and F4/80 are retained, with luciferase activity serving as a direct quantitative measure of IRF pathway activation. The cells are maintained at low passage numbers (<P20) to ensure consistent reporter performance. The cells have undergone rigorous quality control testing and are confirmed free of contamination from mycoplasma, fungi, yeast, and bacteria.