Description
JC-10 is a mitochondrial membrane potential–sensitive fluorescent probe and an improved alternative to JC-1 for detecting changes in mitochondrial membrane potential (ΔΨm). Although JC-1 is widely used, its poor water solubility can cause precipitation in aqueous buffers even at low concentrations, limiting its application. JC-10 exhibits enhanced water solubility and can therefore replace JC-1 in experiments requiring higher dye concentrations. While JC-10 shows superior performance in some cell lines, its effectiveness remains highly cell-type dependent.
In healthy cells, JC-10 selectively accumulates in the mitochondrial matrix and forms reversible aggregates that emit red fluorescence (Ex = 540 nm, Em = 590 nm). When mitochondrial membrane potential decreases or collapses, JC-10 dissociates into monomers that localize in the cytoplasm and emit green fluorescence (Ex = 490 nm, Em = 525 nm).
| Cat.No |
ABC-BR0060
|
| Product Category |
Cell Culture Media, Kits & Reagents |
| Size/Quantity |
100 T
|
| Storage |
-20℃, protect from light, in dry enviornment, avoid repeated freezing and thawing.
|
