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| Product Code | MKN-1; MKN 1 |
| Species | Human |
| Cat.No | ABC-TC0685 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Stomach |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Gastric Cancer Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Due to its rarity, human gastric adenosquamous carcinoma has limited clinical treatment options. The utilization of MKN-1 in the scientific reports might unravel the cell proliferative properties of gastric carcinomas.MKN-1 revealed E-cadherin silencing via promoter CpG hypermethylation.MKN-1 can also used to discover inhibitory effects on tumor invasion. For example, eupatilin and exogenous RUNX3 may inhibit gastric cancer proliferation in MKN-1.
Morphology: MKN-1 cells are epithelial in nature and should appear as adherent, polygonal cells. They should form a monolayer without significant clumping or detachment.
Monitoring: Regularly observe cells under a microscope to ensure they retain their characteristic morphology. Abnormalities in shape or detachment may indicate issues with culture conditions.
Expected Doubling Time: MKN-1 cells typically have a doubling time of 24-36 hours under optimal conditions.
Troubleshooting Slow Growth
Nutrient Depletion: Ensure medium is changed every 2-3 days.
Cell Density: Avoid over-confluency. Split cells when they reach 70-80% confluence.
Contamination: Regularly check for microbial contamination.
Medium Quality: Use high-quality FBS and RPMI 1640 medium. Ensure they are not expired or contaminated.
Incubator Conditions: Confirm stable temperature and CO₂ levels in the incubator.
Viability Assays: Use trypan blue exclusion or MTT assay to determine cell viability.
Morphological Assessment: Regularly observe under a microscope for normal morphology.
Growth Curves: Plot cell growth over time to ensure consistent doubling time and healthy proliferation rates.
