For research use only
| Cat No. | ABC-TC0685 |
| Product Type | Human Gastric Cancer Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Stomach |
| Product Code | MKN-1; MKN 1 |
MKN-1 gastric carcinoma cells with adenosquamous features are used to model tumor progression, epithelial plasticity, and therapy resistance.
The MKN-1 cell line is a poorly differentiated human gastric carcinoma model established from a 61-year-old male patient with poorly differentiated adenocarcinoma. These cells exhibit a unique mixed morphology, featuring both adherent epithelial-like cells and floating signet ring cells, reflecting their origin from a tumor with adenosquamous differentiation. Genetically, MKN-1 harbors mutations in TP53 (R175H) and CDH1 (E-cadherin), showing mixed epithelial-mesenchymal features (co-expression of E-cadherin and vimentin). Additionally, MKN-1 secretes IL-8 and VEGF constitutively, making it valuable for studying tumor angiogenesis and inflammation. MKN-1 cells also express tumor markers such as carcinoembryonic antigen (CEA). These cells are widely used for studies on gastric cancer pathogenesis, drug screening, and metastasis mechanisms.
| Product Code | MKN-1; MKN 1 |
| Species | Human |
| Cat.No | ABC-TC0685 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Stomach |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Gastric Cancer Cell Lines |
MKN-1 is particularly valuable for studying gastric cancer heterogeneity and epithelial-mesenchymal plasticity. Researchers utilize this cell line to investigate diffuse-type gastric cancer progression and chemoresistance mechanisms (5-FU, cisplatin). The mixed phenotype makes it ideal for studying tumor-stroma interactions and metastasis. MKN-1 is also used for developing targeted therapies against HER2 and MET pathways, as well as for gastric biomarker discovery. Its hormone secretion properties support studies of autocrine growth signaling in gastric cancer.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Morphology: MKN-1 cells are epithelial in nature and should appear as adherent, polygonal cells. They should form a monolayer without significant clumping or detachment.
Monitoring: Regularly observe cells under a microscope to ensure they retain their characteristic morphology. Abnormalities in shape or detachment may indicate issues with culture conditions.
Expected Doubling Time: MKN-1 cells typically have a doubling time of 24-36 hours under optimal conditions.
Troubleshooting Slow Growth
Nutrient Depletion: Ensure medium is changed every 2-3 days.
Cell Density: Avoid over-confluency. Split cells when they reach 70-80% confluence.
Contamination: Regularly check for microbial contamination.
Medium Quality: Use high-quality FBS and RPMI 1640 medium. Ensure they are not expired or contaminated.
Incubator Conditions: Confirm stable temperature and CO₂ levels in the incubator.
Viability Assays: Use trypan blue exclusion or MTT assay to determine cell viability.
Morphological Assessment: Regularly observe under a microscope for normal morphology.
Growth Curves: Plot cell growth over time to ensure consistent doubling time and healthy proliferation rates.
