For research use only
| Cat No. | ABC-TC0686 |
| Product Type | Human Gastric Cancer Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Stomach |
| Product Code | MKN28; MKN 28 |
Explore the MKN-28 cell line to model gastric adenocarcinoma and evaluate tumor proliferation, morphology, and chemotherapy responses in vitro.
MKN-28 is a human gastric carcinoma cell line originally established from a metastatic lymph node of a 70-year-old Japanese male patient with well-differentiated intestinal-type adenocarcinoma of the stomach. The cell line exhibits morphological characteristics of intestinal differentiation, including apical-basal polarity and the presence of microvilli with core actin filaments, both in vitro and in xenograft models using nude mice. Genetically, MKN-28 harbors mutations in the TP53 gene (c. 751A>C; p. Ile251Leu) and in the APC tumor suppressor gene. It also shows epigenetic silencing of CD44 and markedly reduced or absent expression of E-cadherin and β-catenin, contributing to impaired cell adhesion and increased malignant potential. The cell line remains tumorigenic in vivo but has not demonstrated metastatic capability. MKN-28 has been identified via STR profiling as a cross-contaminant of MKN-74, according to the International Cell Line Authentication Committee (ICLAC), independent use of the MKN-28 designation should be discontinued, and the line is now referred to as MKN-28/74 in the literature.
| Product Code | MKN28; MKN 28 |
| Species | Human |
| Cat.No | ABC-TC0686 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Stomach |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Gastric Cancer Cell Lines |
The MKN-28 cell line holds significant applications in various areas of research related to human gastric cancer. It is widely used to study gene therapy approaches using exogenous p53, aiming to develop potential treatments for gastric cancer. Additionally, this cell line serves as a valuable model to examine the mechanisms of proliferation and invasion in gastric cancer cells. Moreover, MKN-28 is used to detect microsatellite instability, providing crucial information for making informed therapeutic decisions in the context of gastric cancer treatment strategies.
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