For research use only
| Cat No. | ABC-TC0883 |
| Product Type | Human Ovarian Cancer Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Ovary |
OVMANA is a human ovarian cancer cell line from a Japanese stage IV OCCC patient,expressing HNF1-β and Napsin A, showing tumor growth and drug resistance.
OVMANA is a human ovarian cancer cell line derived from the malignant ascites sample of a Japanese patient diagnosed with stage IV ovarian clear cell adenocarcinoma (OCCC) who had received cisplatin-based chemotherapy. OVMANA exhibits an adherent epithelial morphology and displays characteristics typical of advanced-stage OCCC. These cells harbor several relevant genetic abnormalities, as well as chromosomal instability and aneuploid karyotype. OVMANA cells also express key ovarian cancer markers such as CA125 (MUC16) and PAX8. The cells are tumorigenic in immunocompromised mice, mimicking the aggressive and metastatic nature of HGSOC. This cell line serves as a reliable preclinical model for studying ovarian cancer progression, chemotherapy resistance, and molecular profiling.
| Species | Human |
| Cat.No | ABC-TC0883 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Ovary |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Ovarian Cancer Cell Lines |
OVMANA cells are a critical model for studying ovarian clear cell carcinoma (OCCC), a rare subtype with limited treatment options. They provide insights into OCCC’s molecular and genetic landscape, enabling the screening of therapeutic agents for their efficacy in inducing apoptosis or autophagy. Additionally, OVMANA cells help elucidate mechanisms behind OCCC’s chemoresistance, supporting the development of novel strategies to overcome treatment resistance and improve clinical outcomes.
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OVMANA cells were originally isolated from ovary of a stage IV ovarian tumor patient.
The OVMANA cell line growth rate is poor after thawing, and many dead cells will be observed on the second day. Please try to seed the cells at a higer density. Once attached, the viable cells will grow with doubling time 2-3 days. Complete adherence to the protocol is necessary to ensure the viability of the cells.
1. The recommended culture medium for OVMANA is RPMI-1640 + 10%FBS.
Preheat the complete culture medium in a 37℃ water bath for 30 minutes.
Use a pipette to draw 6-7 mL of complete culture medium into a 15 mL centrifuge tube in a clean bench.
2. Then take the frozen cells out of the liquid nitrogen or dry ice. When reviving, shake them slightly to remove the residual dry ice or liquid nitrogen, then quickly clamp the lid with tweezers and place it in a 37℃ water bath and shake it quickly (note: the water cannot cover the lid), so that it completely melts in about 1 minute.
3. In the clean bench, wipe the outer wall of the cryotube with an 75% alcohol to disinfect it and let it dry slightly. Use a single-channel pipette to transfer all thawed cell suspensions to the complete culture medium prepared in advance, cover the lid, and centrifuge at 1100 rpm for 4 minutes at room temperature to collect cells.
4. Carefully aspirate the supernatant in the clean bench, use a single-channel pipette to absorb 1 mL of fresh complete culture medium to resuspend the cells into a single cell suspension, and then transfer to a T25 cm2 culture flask (or 6cm dish) containing 5 mL of complete culture medium.
Note: Do not directly recover to a T75 cm2 flask or a 10cm dish.
