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| Species | Human |
| Cat.No | ABC-TC0883 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Ovary |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Ovarian Cancer Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
OVMANA cells have significant applications in cancer research. They enhance our understanding of this rare ovarian cancer subtype, which lacks efficient treatment options, through insights into its molecular mechanisms and genetic alterations. OVMANA cells serve as a model for screening therapeutic compounds, evaluating their ability to induce cell death or autophagy in OCCC. This enables the identification of promising candidates for OCCC treatment. Additionally, these cells aid in investigating the high resistance of OCCC to standard chemotherapy, supporting the development of novel therapies to overcome this resistance and improve patient outcomes. In summary, OVMANA cells contribute to understanding OCCC, facilitate drug testing, and advance research on overcoming chemotherapy resistance in OCCC.
OVMANA cells were originally isolated from ovary of a stage IV ovarian tumor patient.
The OVMANA cell line growth rate is poor after thawing, and many dead cells will be observed on the second day. Please try to seed the cells at a higer density. Once attached, the viable cells will grow with doubling time 2-3 days. Complete adherence to the protocol is necessary to ensure the viability of the cells.
1. The recommended culture medium for OVMANA is RPMI-1640 + 10%FBS.
Preheat the complete culture medium in a 37℃ water bath for 30 minutes.
Use a pipette to draw 6-7 mL of complete culture medium into a 15 mL centrifuge tube in a clean bench.
2. Then take the frozen cells out of the liquid nitrogen or dry ice. When reviving, shake them slightly to remove the residual dry ice or liquid nitrogen, then quickly clamp the lid with tweezers and place it in a 37℃ water bath and shake it quickly (note: the water cannot cover the lid), so that it completely melts in about 1 minute.
3. In the clean bench, wipe the outer wall of the cryotube with an 75% alcohol to disinfect it and let it dry slightly. Use a single-channel pipette to transfer all thawed cell suspensions to the complete culture medium prepared in advance, cover the lid, and centrifuge at 1100 rpm for 4 minutes at room temperature to collect cells.
4. Carefully aspirate the supernatant in the clean bench, use a single-channel pipette to absorb 1 mL of fresh complete culture medium to resuspend the cells into a single cell suspension, and then transfer to a T25 cm2 culture flask (or 6cm dish) containing 5 mL of complete culture medium.
Note: Do not directly recover to a T75 cm2 flask or a 10cm dish.
