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| Product Code | RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7 |
| Species | Mouse |
| Cat.No | ABC-TC0934 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Monocyte |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Blood |
| Disease | Leukemia |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Lymphocytes Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
The RAW-264.7 cell line is a widely used model for studying mouse macrophage biology, particularly in immunology and microbiology research. These cells are instrumental in investigating cellular responses to microbial components, inflammation, and immune signaling. This cell line is also extensively used in osteoclast research, as RAW 264.7 cells can differentiate into osteoclast-like cells (OCLs), providing a reliable in vitro system for studying bone metabolism, osteoclastogenesis, and precursor cell migration toward bone. Additionally, RAW-264.7 cells are a preferred choice as a transfection host due to their suitability, making them a versatile tool for exploring gene expression, signal transduction, and host-pathogen interactions across diverse experimental applications.
The RAW 264.7 cells are monocyte/macrophage-like cells. The normal cell morphology generally showed a round and smooth form. A small percentage (<10%) of spindle-shaped or polygonal differentiated cells is considered normal.
Please subculture when density reached 70-80%. Cells should not overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic, prone to differentiate small tentacles.
Cells are semi-adherent, it is recommended to use cell scrapers to gently remove attached cells. Do not use trypsin. Please seed the undifferentiated cells only.
The RAW-264.7 cell seeding is approximate 2-4×10,000 cells/cm². Unlike other cell lines, RAW-264.7 cells do not connect when density is low, instead, they tend to grow in clusters, distributed in patches in the culture vessel.
Induction time and cell density are two key factors related to the differentiation of RAW264.7 cells. It is hard to avoid the differentiation and please control the differentiation ratio into a certain range. Please culture the RAW-264.7 cell line carefully.
Also, RAW264.7 cells might act as osteoclast progenitors and could differentiate to osteoclasts in response to stimulation substances like LPS.
The growth media should be refreshed every 2-3 days, mostly not over 4 days. The average doubling time of RAW cells is 15 hours.
