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RFP-Human Dopaminergic Neuron Cells

Product Name

RFP-Human Dopaminergic Neuron Cells

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Product Code

RFP-HDaNCs

Cat.No

ABC-FC0046

Species

Human

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. RFP-Expressing Human dopaminergic neuronal cells (RFP-HDaNCs) are selected by puromycin after HDaNCs are infected with lentiviruses expressing RFP. Cells are provided with more than 5×10[5] cells/ vial at passage 2. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Product Type

Nervous Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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