For research use only
| Cat No. | ABC-TC1324 |
| Product Type | Human Pancreas Cancer Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Product Code | T3M-4, T3M 4, T3M4 Cells, Human Pancreatic Carcinoma Cell Line T3M-4, Pancreatic Cancer Cells |
T3M-4 pancreatic cancer cells with KRAS, TP53 mutations secrete CEA, tumorigenic with metastatic traits, model pancreatic cancer progression.
The T3M-4 cell line is a human pancreatic ductal adenocarcinoma (PDAC) model derived from a lymph node metastasis. These adherent epithelial cells exhibit a polygonal, cobblestone-like morphology and form monolayers in culture. The cells undergo a more spindle-shaped morphology and TGF-β–dependent epithelial–mesenchymal transition (EMT), characterized by increased vimentin and decreased E-cadherin expression, upon co-culture with activated CD4⁺ T cells. This cell line harbors characteristic PDAC genetic alterations, including mutant TP53 (Y220C) and KRAS mutations (G12D/V/R), making it valuable for studying metastasis, drug resistance (e.g., gemcitabine), and tumor microenvironment interactions. The cell line is also known for its moderate invasiveness and high expression of metastasis-associated genes such as MMPs and CXCR4. Their expression of cancer stemness markers and chemoresistance-associated pathways make them a relevant in vitro model for pancreatic tumor progression and metastasis.
| Product Code | T3M-4, T3M 4, T3M4 Cells, Human Pancreatic Carcinoma Cell Line T3M-4, Pancreatic Cancer Cells |
| Species | Human |
| Cat.No | ABC-TC1324 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Pancreas Cancer Cell Lines |
The T3M-4 cell line is a valuable model for human pancreatic cancer research, enabling gene profiling to analyze oncogene activation, aberrant expression, and epigenetic alterations. It aids in elucidating molecular mechanisms of pancreatic carcinoma. Additionally, T3M-4 cells are used to evaluate the anti-tumor efficacy of antibodies and drugs, supporting the development of novel therapeutic strategies for this malignancy.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Approximately 1-2 ×10^4 cells/cm².
Insufficient Trypsinization: The cells may not have been trypsinized long enough, leading to incomplete detachment and clumping.
Excessive Trypsinization: Prolonged exposure to trypsin can damage cell surface proteins, affecting their ability to reattach.
Incorrect Trypsin Concentration: Using a trypsin solution that is too concentrated can harm the cells, while too dilute may not be effective.
Incomplete Neutralization of Trypsin: If trypsin is not completely neutralized with serum-containing medium after detachment, residual trypsin can continue to damage cells.
Cell Density: Plating cells at too high or too low a density can affect their ability to attach and spread out.
Suboptimal Culture Conditions: Temperature, pH, or medium composition might not be optimal for cell attachment.
Optimize Trypsinization Time: Ensure cells are exposed to trypsin-EDTA for an appropriate duration (usually 2-5 minutes at 37°C) to detach without excessive damage. Monitor under a microscope.
Neutralize Trypsin Effectively: Immediately add an appropriate amount of medium containing serum to neutralize the trypsin after detachment.
Use Gentle Pipetting: Gently pipette the cells up and down to break up clumps without causing excessive mechanical damage.
Optimize Seeding Density: Seed cells at an appropriate density (e.g., 1-2 x 10^4 cells/cm²) to allow for optimal attachment and growth.
Check Culture Conditions: Ensure the culture medium is pre-warmed to 37°C and that the CO₂ levels and pH are appropriate.
Coating Culture Vessels: If cells still do not attach well, consider coating the culture vessels with extracellular matrix proteins (e.g., collagen, fibronectin) to enhance cell adhesion.
