T84 Luciferase Reporter Cell Line

Frequently Asked Questions

• What is the T84 Luciferase Reporter Cell Line?

  The T84 Luciferase Reporter Cell Line is generated from the T84 colon epithelial cell line, which has been modified to constitutively express the luciferase gene. This allows for the real-time monitoring of cellular processes using luminescence as a reporter, making it a valuable tool for research in cellular signaling, drug discovery, and understanding colorectal carcinoma mechanisms.

• What are the growth and maintenance conditions for the T84 Luciferase Reporter Cell Line?

  The T84 Luciferase Reporter Cell Line should be cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics-antimycotics. For selection and maintenance of the luciferase-expressing cells, 1 µg/mL puromycin can be added to the culture medium.

• How should the T84 Luciferase Reporter Cell Line be thawed and processed?

  The T84 Luciferase Reporter Cell Line should be thawed rapidly in 37°C, ensuring that the vial is kept out of the water to avoid contamination. After thawing, these cells should be transferred to a centrifuge tube containing 5 mL of complete culture medium, centrifuged at 200×g for 5-10 minutes, and the supernatant discarded. The cell pellet should be resuspended in fresh medium and transferred to a new culture flask.

• What are the specific applications of the T84 Luciferase Reporter Cell Line?

  The T84 Luciferase Reporter Cell Line is particularly useful for studies involving gene expression regulation, cellular responses to pharmacological agents, and molecular pathways involved in colorectal cancer. The cell line’s stable expression of luciferase allows for convenient and effective bioluminescent imaging, facilitating in vivo tracking of tumor growth and response to treatments in research models.

• What are the safety levels and precautions associated with handling this cell line?

  The T84 Luciferase Reporter Cell Line is classified as biosafety level 1. Appropriate safety procedures should be followed when handling this cell line, including the use of personal protective equipment. The cells should be handled under biological safety cabinet conditions to prevent contamination and ensure user safety.