For research use only
| Cat No. | ABC-TC1338 |
| Product Type | Human Liver Cell Lines |
| Cell Type | Epithelial |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Liver |
| Disease | Normal |
| Product Code | THLE3; Transformed Human Liver Epithelial-3 |
THLE-3 human liver epithelial cells immortalized by SV40, metabolically active,non-tumorigenic, near-diploid,ideal for liver toxicity and drug metabolism.
THLE-3 is a human liver epithelial cell line derived from the left lobe of a healthy adult donor and immortalized by SV40 large T antigen. The SV40 large T antigen mediates immortalization through inactivation of the p53 and Rb tumor suppressor pathways, thereby overriding cell cycle checkpoints and enabling extended proliferative capacity. THLE-3 cells retain phenotypic features of normal adult hepatocytes, exhibit a near-diploid karyotype, and lack expression of alpha-fetoprotein (AFP). They are non-tumorigenic and non-metastatic in athymic nude mice. Functionally, THLE-3 cells maintain active cytochrome P450 enzymatic pathways and efficiently metabolize procarcinogens such as benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 into DNA-adduct forming intermediates. Aside from SV40-mediated transformation, these cells do not harbor known oncogenic mutations or overexpression. THLE-3 serves as a reliable in vitro model for liver toxicology, drug metabolism, and xenobiotic biotransformation studies.
| Product Code | THLE3; Transformed Human Liver Epithelial-3 |
| Species | Human |
| Cat.No | ABC-TC1338 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Liver |
| Disease | Normal |
| Biosafety Level | 2 |
| Storage | Liquid Nitrogen |
| Product Type | Human Liver Cell Lines |
THLE-3 cells are used in pharmacotoxicological research and hepatocellular carcinoma pathogenesis studies. As immortalized human liver cells, they offer a reliable in vitro model for liver toxicity and cancer research. Their application lies in being a normal cell model for various assays, enabling comparison of responses to compounds between healthy and cancerous liver cells, ensuring that potential effects of substances are specifically targeted towards diseased cells while leaving healthy cells unaffected.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
