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Transfected Stable Cell Lines

THP-1/NFκB & IRF Reporter Cell Line

  • For research use only

Cat No.

ABC-RC040Y
Product Type

Reporter Stable Cell Lines
Cell Type

Monocyte
Species

Human
Host Cell

THP-1
Source Organ

Peripheral Blood
Disease

Acute Monocytic Leukemia
Storage

Liquid Nitrogen

THP-1/NFκB & IRF Reporter Cell Line by AcceGen enables dual-pathway monitoring of innate immunity via NFκB-SEAP and IRF-Lucia bioluminescent assays.

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  • THP-1/NFκB & IRF Reporter Cell Line
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Home | Transfected Stable Cell Lines | THP-1/NFκB & IRF Reporter Cell Line

Description

THP-1/NFκB & IRF Reporter Cell Line is a dual-reporter human monocytic model derived from THP-1 cells, engineered to monitor NFκB (nuclear factor kappa-light-chain-enhancer of activated B cells) and IRF (interferon regulatory factor) signaling pathways simultaneously. This cell line expresses secreted alkaline phosphatase (SEAP) under NFκB response elements and Lucia luciferase under IRF-responsive promoters, allowing sensitive and quantitative dual readouts of inflammatory and interferon signaling. It retains key THP-1 features, including CD14 upregulation upon differentiation and PMA-induced macrophage-like conversion, making it particularly valuable for studies of innate immune activation, pathogen recognition, vaccine adjuvant screening, and immunomodulatory drug development. Cells are cryopreserved at low passage (<P20) to ensure stable reporter performance and reproducibility. All batches are rigorously tested and confirmed free of HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.

Species

Human
Cat.No

ABC-RC040Y
Product Category

Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Monocyte
Growth Mode

Suspension
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Peripheral Blood
Disease

Acute Monocytic Leukemia
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Reporter Stable Cell Lines
Host Cell

THP-1
Gene Info

NFκB–SEAP and IRF–Lucia / Luciferase
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • The THP-1/NFκB & IRF Reporter Cell Line is a dual-pathway human monocytic model engineered to simultaneously monitor both NFκB and IRF signaling activation upon TLR or cytokine stimulation. This system enables parallel quantification of inflammatory (NFκB) and interferon (IRF) responses through dual-reporter outputs, making it ideal for studying innate immunity crosstalk, vaccine adjuvant screening, and autoimmune/inflammatory disease mechanisms. Maintain in RPMI-1640 with 10% FBS and selection antibiotics to ensure stable reporter activity during THP-1 differentiation and stimulation experiments.

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

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