| Species |
Human
|
| Cat.No |
ABC-RC050Y
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Transfected Stable Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
Monocyte
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Peripheral Blood
|
| Disease |
Acute Monocytic Leukemia
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Reporter Stable Cell Lines
|
| Host Cell |
THP-1
|
| Gene Info |
SMAD / TGF β / Luciferase
|
Description
THP-1 SMAD/TGF β Reporter (Luc) Stable Cell Line is a genetically engineered human monocytic cell line designed to monitor SMAD-dependent TGF β (Transforming Growth Factor Beta) signaling through luciferase (Luc) reporter expression. Derived from THP-1 cells, the cell line maintains key native THP-1 characteristics, such as the capacity to upregulate CD14 upon differentiation and PMA-induced differentiation into macrophage-like cells while featuring a luciferase reporter system under the control of SMAD-responsive elements. The cell line enables sensitive quantification of TGF β pathway activation through bioluminescence measurement, making it particularly valuable for studying immune regulation, fibrosis mechanisms, and cancer microenvironment research. Cells exhibit dose-dependent luciferase activity upon TGF β stimulation while retaining their original biological properties, allowing for high-throughput drug screening and mechanistic studies of SMAD signaling dynamics. Maintained at low passage numbers (<P20) to ensure reporter stability and consistent performance, this cell line provides a reliable tool for immunology and translational medicine research. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, Fungi, Yeast and Bacteria.
