MicroRNA Agomir/Antagomir

About MicroRNA Agomir/Antagomir

miRNA Tools for Post-Transcriptional Gene Regulation

MicroRNA (miRNA) is a type of endogenous small RNA with a length of about 20-24 nucleotides. A combination of several miRNAs can be used to fine-tune the expression of a gene. MicroRNA Agomir is a special-labeled and chemically modified double-stranded small RNA that mimics the endogenous miRNA to regulate the biological function of the target gene. MicroRNA Antagomir is an especially chemically modified miRNA antagonist. It strongly competes with mature miRNAs in the body to prevent the complementary pairing of miRNAs and their target genes, thereby inhibiting miRNAs from functioning.

NOMENCLATURE

1. The miRNA mature body is abbreviated as miR, and then according to its species name, such as the human being abbreviated as hsa, and the order in which it was found is represented by Arabic numerals, such as hsa-miR-122.
2. Highly homologous miRNAs are followed by lowercase letters (a, b, c, ...), such as hsa-miR-34a, hsa-miR-34b, hsa-miR-34c, and so on.
3. MiRNAs with the same mature sequence are transcribed and processed from DNA sequences on different chromosomes, followed by Arabic numerals to indicate the difference, such as hsa-miR-199a-1 and hsa-miR-199a-2.
4. Generally, a miRNA precursor is about 70-80nt in length. It is likely that miRNAs are generated by the two arms, and they are named by "-5p" and "-3p". For example, hsa-miR-26b-5p and hsa-miR-26b-3p indicate that they are processed from the 5 'end arm and 3' end arm of the hsa-mir-26b precursor, respectively.

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MicroRNA Agomir/Antagomir

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