Technical Support
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Hot Products
- In-Stock Tumor Cell Lines
- Human Orbital Fibroblasts
- Human Microglia
- Human Pulmonary Alveolar Epithelial Cells
- Human Colonic Fibroblasts
- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
- Human Thyroid Epithelial Cells
- C57BL/6 Mouse Dermal Fibroblasts
- Human Alveolar Macrophages
- Human Dermal Fibroblasts, Adult
- Human Lung Fibroblasts, Adult
- Human Retinal Muller Cells
- Human Articular Chondrocytes
- Human Retinal Pigment Epithelial Cells
- Human Pancreatic Islets of Langerhans Cells
- Human Kidney Podocyte Cells
- Human Renal Proximal Tubule Cells
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Can primary cells be passaged?
Most primary cells have a limited proliferative capacity and can only be passaged for a few generations, depending on the specific cell type. With each passage, primary cells gradually lose their original biological functions. Therefore, it is recommended to use the cells within the suggested passage range. Terminally differentiated primary cells cannot be expanded or passaged.
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What is the passage limit and doubling time of Human Preadipocytes-Subcutaneous under standard culture conditions?
Human Preadipocytes-Subcutaneous can be passaged up to 4 times, with a doubling time of approximately 24-48 hours. However, the specific conditions depend on experimental procedures, cell status, and culture conditions.
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What is the source of Human Oral Epithelial Cells?
AcceGen's Human Oral Epithelial Cells are mainly isolated from the gingival mucosa and buccal mucosa of human oral cavity.
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What is the source of Human PB Red Blood Cells/Mature Erythrocytes?
The peripheral blood is collected by leukapheresis from fully consented IRB approved donors who are tested negative for HIV, HBV, and HCV. A common anticoagulant acid-citrate-dextrose formula A (ACD-A) is used to prevent the blood from clotting during the leukapheresis process.
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What is the passage limit for Human Lobar Bronchial Epithelial Cells?
These cells are typically viable for 10-12 passages before cellular senescence.
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What lymphocyte types are present in Human Spleen Cells?
Human Spleen Cells contain T cells, B cells, and NK cells. Among them, T cells and B cells are present in higher proportions, while NK cells are less abundant.
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Do Human Ovarian Surface Epithelial Cells require gelatin coating?
These primary cells adhere poorly at the begining, so gelatin coating is recommended. In later passages, coating might be reduced or omitted.
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What is the species information and coating concentration of collagen required for Human Microglia?
The Type I collagen provided by AcceGen is purified from rat tail tendons. The recommended coating concentration is 0.5 μg/cm².
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What is the passage limit and doubling time for Human Corneal Keratocytes?
Human Corneal Keratocytes can be passaged 12-15 times, with a doubling time of approximately 24-36 hours. However, the specific conditions may vary depending on experimental procedures, cell status, and culture conditions.
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How are Human Esophageal Epithelial Cells obtained?
These cells are isolated from human esophageal tissue using trypsin digestion.
