0.4% Trypan Blue Solution (0.4%)

Application

• Trypan Blue solution is used as a cell stain to assess cell viability using the dye exclusion test, where viable cells do not take up the dye. Trypan blue can also stain collagen and amyloid proteins. The complexes formed by trypan blue and protein emit red fluorescence. Furthermore, trypan blue (at appropriate concentrations) is often used to quench cell surface autofluorescence or other fluorescent signals.