B6129SF2/J Mouse Splenic Fibroblasts

Description

B6129SF2/J Mouse Splenic Fibroblasts are primary cells isolated from the spleen tissue of B6129SF2/J hybrid mice. These fibroblasts, characterized by their spindle-shaped morphology and adherence to culture surfaces, are non-hematopoietic stromal cells that play a structural and immunomodulatory role within the splenic microenvironment. They support the organization of splenic architecture and interact with immune cells to influence immune homeostasis and inflammation. Studies have shown that splenic fibroblasts are involved in regulating lymphocyte positioning and antigen presentation indirectly through chemokine expression. These cells exhibit spindle-shaped morphology and adhere to culture surface. These cells typically exhibit stable karyotypes and can express markers such as PDPN and CD90. They are cryopreserved at early passage post-isolation to maintain physiological relevance. These cells are not immortalized and have limited proliferative capacity in vitro. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.