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Tumor Cell Lines

B95-8

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Cotton-top Tamarin Monkey peripheral blood lymphocyte. Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming EBV. Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells.
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Product Code

B95.8; B 95.8; B 95-8; B-95-8; B958

Species

Saguinus oedipus

Cat.No

ABC-TC0065

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Fibroblast

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Blood

Biosafety Level

2

Storage

Liquid Nitrogen

Product Type

Monkey Lymphocytes Cell Lines

Description

B95-8The B95-8 cell line was established in 1973 from the peripheral blood lymphocytes of a cotton-top tamarin (Saguinus oedipus), transformed by Epstein-Barr virus (EBV). It is widely used to produce high titers of infectious EBV, facilitating the generation of EBV-transformed human B cell lines. B95-8 cells grow predominantly in suspension with round to oval morphology, occasionally forming small clumps, and around 10% of the cells show slight adherence. These cells carry a near-diploid simian karyotype with chromosomal abnormalities and are EBV-positive but free of HIV-1, HIV-2, HTLV-I/II, HBV, HCV, and CMV. Some batches may contain Type D retrovirus, so screening is recommended. B95-8 cells are responsive to B cell factors like sCD23 and are commonly used in virology and lymphocyte transformation research.

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Citation

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Application

  • The B95-8 cell line is a classic model for EBV research, used to reveal the latent infection mechanism of EBV, the oncogenic effects of viral genes (such as LMP-1 and EBNA-2), and the interaction between the virus and host cells. In addition, B95-8 cells can continuously secrete infectious EBV particles, so they are often used as a tool for EBV virus amplification to provide a virus source for the laboratory.

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