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BALB/c Mouse Mammary Fibroblasts

Product Name

BALB/c Mouse Mammary Fibroblasts

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Cat.No

ABC-TC3091

Species

BALB/c Mouse

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

BALB/c Mouse Primary Mammary Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Mammary Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
Mouse Primary Mammary Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Disease

Normal

Source Organ

Mammary

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Fibroblast

Growth Mode

Adherent

Product Type

Mouse Primary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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