C57BL/6 Mouse Cardiac Microvascular Endothelial Cells

Description

C57BL/6 Mouse Cardiac Microvascular Endothelial Cells (CMECs) are primary cells isolated from the myocardial microvasculature of pathogen-free laboratory mice. They reside in the capillary network of the cardiac microvasculature, embedded within the myocardium between cardiomyocyte bundles. Following primary culture, these cells are cryopreserved. These cardiac endothelial cells govern myocardial perfusion by regulating capillary tone via vasoactive molecules, maintain microvascular barrier integrity through junctional proteins (e.g., claudin-5), and mediate cardiomyocyte-endothelial crosstalk for metabolic exchange. CMECs express VE-cadherin, vWF, and CD31/PECAM-1. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.