C57BL/6 Mouse Embryonic Fibroblasts

Description

C57BL/6 Mouse Embryonic Fibroblasts are primary cells isolated from the trunk, limb buds, and head mesenchyme of embryonic pathogen-free laboratory mice. These cells primarily reside within the embryonic connective tissue matrix. Following primary culture, these cells are cryopreserved. These cells play a crucial role in supporting pluripotent stem cell maintenance through extracellular matrix secretion and paracrine signaling. Studies have demonstrated their involvement in premature aging disorders, cellular reprogramming defects for neurodegenerative disease modeling, and radiation-induced genomic instability. The cells exhibit a characteristic spindle-shaped morphology and are validated by the fibroblast-specific protein 1 (FSP1/S100A4). For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.