CD-1 Mouse Embryonic Fibroblasts

Description

CD-1 Mouse Embryonic Fibroblasts (MEFs) are primary fibroblasts isolated from embryos of healthy, pathogen-free CD-1 mice using enzymatic digestion and mechanical dissociation to preserve viability and native function. MEFs exhibit typical spindle-shaped morphology, adhere robustly, and proliferate well under defined culture conditions, providing a valuable model for studying cell signaling pathways in CD-1 mice. They serve as a physiologically relevant feeder layer by secreting extracellular matrix proteins and growth factors essential for maintaining mouse and human pluripotent stem cell pluripotency. MEFs serve as reliable, high-quality support cells for stem cell research and related applications. To ensure the quality of these primary cells, repeated freeze-thawcycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.