CF1 Mouse Embryonic Fibroblasts

Description

CF1 Mouse Embryonic Fibroblasts (MEFs) are isolated from CF1 mice. These primary fibroblasts are adherent cells with a flattened morphology. MEFs play a critical role in the formation of the extracellular matrix and support tissue architecture during embryogenesis. After isolation, the cells are typically cryopreserved at early passage to maintain their primary characteristics. CF1 MEFs are routinely used as feeder layers for maintaining the pluripotency of mouse and human embryonic stem cells. Studies have shown that MEFs can secrete growth factors and cytokines involved in wound healing, inflammation, and cell differentiation processes, including TGF-β and FGF2. These cells express markers such as vimentin and fibroblast-specific protein-1 (FSP1), verified by immunocytochemistry. To preserve cellular integrity, repeated freeze-thaw cycles should be avoided. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.