FCGR1A (CD64) Knockout THP-1 Cell Line

Description

The FCGR1A (CD64) Knockout THP-1 Cell Line is a CRISPR/Cas9-engineered human monocytic model derived from THP-1 cells, featuring targeted disruption of the Fc gamma receptor Ia (FcγRI/CD64) gene. This high-affinity IgG receptor is a key mediator of antibody-dependent cellular phagocytosis (ADCP) and immune complex clearance. Loss of CD64 expression results in impaired Fcγ receptor signaling, while the cells retain hallmark THP-1 properties including suspension growth, round morphology, and PMA-induced differentiation capacity. This knockout system provides a powerful tool for mechanistic studies of Fc receptor function, autoimmune disease modeling, and evaluation of therapeutic antibody efficacy. Maintained at low passage numbers (<P20) to ensure genetic stability, the knockout is validated by genomic PCR, sequencing, and flow cytometry. Each batch is rigorously tested to confirm absence of HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.