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GFP-Mouse Heart Microvascular Endothelial Cells

Product Name

GFP-Mouse Heart Microvascular Endothelial Cells

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Product Code

mHtMECs-GFP

Cat.No

ABC-FC0055

Species

Mouse

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Mouse Heart Microvascular Endothelial cells (mHtMECs) are isolated from hearts of 3-week old male C57BL/6 mice and GFP expressing mHtMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with > 5 x 105 cells/vial). mHtMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mHtMECs is not recommended, even cultured following the detailed protocol described below.

Disease

Normal

Source Organ

Heart

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

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Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Endothelial

Growth Mode

Adherent

Product Type

Mouse Primary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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