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Human Oct4 EGFP Reporter Cell Line-H1 |
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Product Name |
Human Oct4 EGFP Reporter Cell Line-H1 |
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Price | Get Quote | |||
Cat.No |
ABC-RC232D |
Species |
Human |
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Size/Quantity |
1 vial |
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Shipping Info |
Dry Ice |
Storage |
Liquid Nitrogen |
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Description |
GFP expression linked directly to the Oct4 promoter. A GFP Neomycin construct was added to the H1 cell line via homologous recombination and targeted to the POUF1 gene. Cells will express GFP when Oct4 is expressed (e.g. undifferentiated state). Oct4/GFP positive cells are selectable using Neomycin. These cells tend to differentiate more frequently and are best grown in slightly sparser conditions. It is not unusual to have to manually remove differentiation at each passage in the absence of selection. |
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Recommended Medium And Supplement | Thaw Medium: MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% peillin G/Streptomycin. Growth Medium: Thaw Medium 1 plus 400 μg/ml of Geneticin. |
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Citation Guide |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). |
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Host Cell | H1 |
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Application | For research use only |
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Growth Conditions | 37 ℃, 5% CO2 |
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Growth Mode | Adherent |
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Product Type |
Reporter Stable Cell Lines |
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Product Image |
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