GFP expression linked directly to the Oct4 promoter. A GFP Neomycin construct was added to the H1 cell line via homologous recombination and targeted to the POUF1 gene. Cells will express GFP when Oct4 is expressed (e.g. undifferentiated state). Oct4/GFP positive cells are selectable using Neomycin. These cells tend to differentiate more frequently and are best grown in slightly sparser conditions. It is not unusual to have to manually remove differentiation at each passage in the absence of selection.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
For research use only
Reporter Stable Cell Lines
- ONLINE INQUIRY
- PRODUCT REVIEWS
Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.