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Transfected Stable Cell Lines

Xpress™ FcRn (FCGRT & B2M), GFP Tag Over-expressing Stable Cell Line (HEK-293)

  • For research use only

Cat No.

ABC-RC022F
Product Type

Reporter Stable Cell Lines
Cell Type

Epithelial
Species

Human
Host Cell

HEK293
Source Organ

Kidney
Disease

Normal
Storage

Liquid Nitrogen

Xpress™ FcRn (FCGRT & B2M), GFP Tag Over-expressing Stable Cell Line (HEK-293) stably expresses FcRn with GFP for functional monoclonal cell studies.

Product Image
  • Brightfield micrograph of FcRn-FCGRT-B2M-GFP-Over-expressing-HEK-293, adherent growth, 10X mag.
  • Xpress™ FcRn (FCGRT & B2M), GFP Tag Over-expressing Stable Cell Line (HEK-293)
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Home | Transfected Stable Cell Lines | Reporter Stable Cell Lines | Xpress™ FcRn (FCGRT & B2M), GFP Tag Over-expressing Stable Cell Line (HEK-293)

Description

A monoclonal cell line stably expressing FcRn (FCGRT & B2M), GFP Tag was constructed using viral technology based on HEK-293 cells.

Species

Human
Cat.No

ABC-RC022F
Product Category

Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Epithelial
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Kidney
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Reporter Stable Cell Lines
Host Cell

HEK293
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Frequently Asked Questions

  • How is the Xpress™ FcRn (FCGRT & B2M), GFP Tag Over-Expressing Stable Cell Line (HEK-293) constructed, and is it suitable for FcRn-mediated recycling studies?

    The Xpress™ FcRn (FCGRT & B2M), GFP Tag Over-Expressing Stable Cell Line (HEK-293) is engineered to stably express human FCGRT (FcRn α-chain) and B2M (β2-microglobulin), which together form the FcRn complex. GFP is expressed as a screening marker for identifying positive cells by flow cytometry.

     

    The current characterization data are based on internal quality control analysis. Although FcRn expression in heterologous cell systems may support studies of FcRn-related mechanisms, functional validation of FcRn-mediated recycling activities, such as pH-dependent binding and intracellular recycling assays, has not been performed for this cell line.

  • What are the recommended culture conditions and maintenance guidelines for the Xpress™ FcRn stable cell line

    The cells are recommended to be cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, Zeocin (25 μg/mL), and Puromycin (5 μg/mL) at 37°C with 5% CO₂.

     

    The expected doubling time is approximately 22–24 hours under recommended conditions. Cells are typically passaged at a ratio of 1:3 to 1:4, and the corresponding selection reagents should be maintained during routine culture to support stable expression.

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