HighQC™ CHOPWT9 Induced Pluripotent Stem Cell

Description

HighQC™ CHOPWT9 Induced Pluripotent Stem Cells are generated from peripheral blood mononuclear cells collected from a healthy adult female and reprogrammed into a pluripotent state using Sendai virus–mediated delivery of the transcription factors OCT3/4, SOX2, c-MYC, and KLF4. Morphologically, these cells typically grow as compact, colony-forming cells with well-defined borders and exhibit features associated with human pluripotent stem cells, including relatively large nuclei, limited cytoplasm, and a high nuclear-to-cytoplasmic ratio. They express pluripotency-associated markers, with SSEA-4 commonly used for positive identification as assessed by immunofluorescence staining or flow cytometry, and demonstrate the capacity for differentiation into all three germ layers—ectoderm, mesoderm, and endoderm—under appropriate induction conditions. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.