Human Ankylosing Spondylitis Peripheral Blood Mononuclear Cells

Description

Human Ankylosing Spondylitis Peripheral Blood Mononuclear Cells (AS PBMCs) are isolated from peripheral blood of patients with clinically confirmed AS, a chronic inflammatory arthritis primarily affecting the axial skeleton. Peripheral ankylosing spondylitis represents a clinical manifestation in which immune dysregulation extends beyond axial involvement and can be evaluated using disease-derived PBMCs. These human-derived PBMCs contain immune cell populations (Th17 cells, γδ T cells, and monocytes) that demonstrate AS-specific immune activation pattern, including elevated IL-17/IL-22 cytokine production, and show expression of HLA-B27 heavy chain homodimers mainly on antigen-presenting and myeloid cells. The cells exhibit typical mononuclear morphology with intact surface markers and maintain functional responses to bacterial antigens, reflecting the proposed gut-joint axis in AS pathogenesis. These PBMCs are particularly valuable for studying the interplay between genetic factors, microbial triggers, and innate immune activation that drives AS progression.