Human Bladder Fibroblasts, Secondary

Description

Human Bladder Fibroblasts, Secondary are primary cells isolated from human bladder tissue. They are typically obtained from non-diseased bladder samples and cryopreserved at a secondary passage to retain characteristic functions. They exhibit spindle-shaped morphology and adherent growth in a monolayer. Functionally, they contribute to the synthesis and maintenance of the bladder’s extracellular matrix for structural support, and serve as a valuable in vitro model for studying fibroblast involvement in bladder cancer, while their dysregulation is linked to bladder fibrosis and other bladder-related connective tissue disorders. These fibroblasts express vimentin. They can be passaged multiple times under appropriate cell-culture conditions. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.​