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Human Endometriotic Stromal Cells

Product Name

Human Endometriotic Stromal Cells

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Cat.No

ABC-TC069G

Species

Human

Cell Type

Stromal Cell

Growth Mode

Adherent

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

AcceGen provides high-quality Human Endometriotic Stromal Cells from Endometriosis Tissue, which are cryopreserved at passage 1 with 0.5 million cells in each vial. Human Endometriotic Stromal Cells express Vimentin and can be further expanded for 6-8 passages under the conditions suggested by AcceGen.

Disease

Endometriosis

Source Organ

Endometriosis Tissue

Quality Control

All cells test negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

QuestionsthreeWhat are the optimal growth conditions for culturing Human Endometriotic Stromal Cells?
Product Type

Female Reproductive Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial

Frequently Asked Questions

  • What are Human Endometriotic Stromal Cells?

    Human Endometriotic Stromal Cells are derived from the stromal tissue of endometriotic lesions. These cells are isolated from surgically obtained endometriotic tissue samples, ensuring high purity and viability for research applications.

  • What are the primary applications of Human Endometriotic Stromal Cells in research?

    These cells are extensively used in studies focusing on endometriosis pathogenesis, drug screening, and the development of novel therapeutic strategies. They are also valuable for investigating the cellular and molecular mechanisms underlying endometriotic lesion formation and progression.

  • What are the optimal growth conditions for culturing Human Endometriotic Stromal Cells?

    Human Endometriotic Stromal Cells require a specialized growth medium designed for stromal cells, typically supplemented with fetal bovine serum, growth factors, and other essential nutrients. Cells should be cultured at 37°C in a humidified atmosphere with 5% CO2.

  • What are the key characteristics and markers of Human Endometriotic Stromal Cells?

    These cells exhibit fibroblast-like morphology and express specific markers such as vimentin, CD10, and estrogen receptors. They are also characterized by their ability to respond to hormonal stimuli, which is crucial for studying endometriosis.

  • How should Human Endometriotic Stromal Cells be handled during passaging to ensure optimal cell state?

    When passaging these cells, it is important to avoid over-confluency, as it can affect their growth and viability. Typically, cells should be passaged at 70-80% confluency using standard trypsinization protocols. Ensuring gentle handling and using appropriate cell culture reagents will help maintain cell integrity and functionality.

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